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Jul 21, 2009·The kinase reaction was carried out by incubating 3 μM tEGFR-EGF complex in 15μl reaction buffer (20 mM Hepes, pH 7.4, 1 mM MnCl 2, 5 mM MgCl 2, 1 mM ATP, 1 mM Na 3 VO 4) for 30 min at 30°C. The reaction was stopped by the addition of SDS gel loading buffer, the proteins were resolved on SDS-PAGE.

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Delivery of GABAARs to Synapses Is Mediated by HAP1-KIF5 ...

Electrodes were filled with the following internal solution: 100 mM CsCl, 30 mM N-methyl-D-glucamine, 10 mM HEPES, 1 mM MgCl 2, 1 mM EGTA, 5 mM QX314, 12 mM phosphocreatine, 5 mM MgATP, 1.5 mM Na 2 GTP, 0.1 mM leupeptin [pH = 7.2–7.3], 265–270 mOsm/l. The neurons were recorded in tetrodotoxin (0.5 μM) and held at −70 mV.

Delivery of GABAARs to synapses is mediated by HAP1-KIF5 ...

Jan 01, 2010·Electrodes were filled with the following internal solution: 100 mM CsCl, 30 mM N-methyl-D-glucamine, 10 mM HEPES, 1 mM MgCl 2, 1 mM EGTA, 5 mM QX314, 12 mM phosphocreatine, 5 mM MgATP, 1.5 mM Na 2 GTP, 0.1 mM leupeptin, pH = 7.2–7.3, 265– 270 mOsm/liter. The neurons were recorded in tetrodotoxin (0.5 µM) and held at −70mV.

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GlobalFoundries - Wikipedia

Fab 1 . Fab 1, located in Dresden, Germany, is a 364,512 m 2 plant which was transferred to GlobalFoundries on its inception: Fab 36 and Fab 38 were renamed Module 1 and Module 2, respectively. Each module can produce 25,000 300 mm diameter wafers per month.

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Nanoprobes e-News Vol. 8 No. 3 (March 13, 2007)

Next, the sections were incubated in a mixture of goat Nanogold-Fab' anti-rabbit affinity-purified Fab' fragments (1: 100). Gold particles were enhanced by silver amplification for 8 to 12 minutes with HQ Silver. Sections were treated with 1% OsO 4 and contrasted in 1% uranyl acetate before embedding. Serial electron microscopic sections were ...

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Delivery of GABAARs to synapses is mediated by HAP1-KIF5 ...

Jan 01, 2010·Electrodes were filled with the following internal solution: 100 mM CsCl, 30 mM N-methyl-D-glucamine, 10 mM HEPES, 1 mM MgCl 2, 1 mM EGTA, 5 mM QX314, 12 mM phosphocreatine, 5 mM MgATP, 1.5 mM Na 2 GTP, 0.1 mM leupeptin, pH = 7.2–7.3, 265– 270 mOsm/liter. The neurons were recorded in tetrodotoxin (0.5 µM) and held at −70mV.

Textron

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